Optimization of flavonoids extraction from the leaves of Tabernaemontana heyneana Wall

Abstract: The study has been carried out to investigate the effects of single factors such as temperature, extraction time, concentration of ethanol, material ratio and no. of extractions on the contents of flavonoids present in the leaves of Tabernaemontana heyneana Wall. were investigated in this study. On this basis, an L16 orthogonal design of experiment was adopted to determine the optimal conditions for the extraction of flavonoids. The amount of flavonoids extracted reached its maxima when extracted at 85oC for 2hrs by using 75%ethanol with a material ratio of 1:05 and 4 times of extraction. The TLC performed for the optimized extracts showed the presence of rutin, quercetin related compounds. The PTLC of the optimized extracts also proved the presence of flavonoids, especially high levels of rutin related compounds. [Nature and Science. 2008;6(3):10-21]. ISSN: 1545-0740.